Identifying CTX-M Resistance Gene Beta-lactamase in the Separated Escherichia Coli from Urine Samples by Polymerase Chain Reaction (PCR) in Khoramabad, Iran

Abstract

The most important factor in resistance to cephalosporin is Escherichia coli bacteria producing Extended SpectrumBeta Lactamases (ESBLs). Over the past decade, CTX-M enzymes have been the most common type of broad-spectrum beta-lactamases in Europe, Canada and Asia. Therefore, the purpose of the research was to study the prevalence of E. coli bacteria producing ESBL and CTX-M-I group with molecular method. There were totally gathered 257 urine samples from 12 medical laboratories in Khoramabad. After culturing them on EMB agar environment at 37°C for 24 h and biochemical testing for confirmation, there were isolated 100 samples of 257 samples of Escherichia coli. Presence of CTX-M gene was studied by PCR method on the separated isolates in diagnostic tests of Diffusion agar disk and combined disk. There were 31 (31%) strains of ESBL producing out of 100 examined strains. PCR process to detect CTX-M genes showed that 16 out of 31 strains of ESBL (51.161%) contained the considered gene. The results of this study show high percentage of beta-lactamases resistant among E. coli strains. This indicates a serious public danger that all measures must be taken to avoid this danger.